Journal: Frontiers in Veterinary Science

Article Title: MNQ derivative D19 alleviates LPS-induced inflammation and oxidative stress in sheep follicular granulosa cells through the GPX4 -mediated ferroptosis

doi: 10.3389/fvets.2025.1621738

Figure Lengend Snippet: D19 restoring LPS-suppressed steroid hormone production in GCs. (A) ELISA measurement of A 4 , E 2 , and P 4 levels in GCs. (B,C) qRT-PCR and Western blotting were employed to detect the relative expression levels of mRNA and protein, respectively, for genes involved in steroid hormone synthesis ( HSD17B4 , CYP19A1 , 3β-HSD , CYP11A1 , and STAR ). Data from at least three independent experiments are presented as mean ± SEM. Significant differences ( p < 0.05) are denoted by different letters.

Article Snippet: CYP19A1 , bs-0114R , Bioss , Rabbit , 1:500.

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Expressing

Journal: Frontiers in Veterinary Science

Article Title: MNQ derivative D19 alleviates LPS-induced inflammation and oxidative stress in sheep follicular granulosa cells through the GPX4 -mediated ferroptosis

doi: 10.3389/fvets.2025.1621738

Figure Lengend Snippet: D19 preventing GPX4 deficiency-induced disruption of steroidogenesis in GCs. (A) Levels of E 2 and P 4 in different treatment groups were measured using ELISA. (B) The relative mRNA and protein expression levels of steroid hormone synthesis-related genes ( HSD17B4 , CYP19A1 , 3β-HSD , CYP11A1 , and STAR ) were detected by qRT-PCR and Western blotting, respectively. All experiments were performed in triplicate, and data are presented as mean ± SEM. Different letters indicate statistically significant differences ( p < 0.05).

Article Snippet: CYP19A1 , bs-0114R , Bioss , Rabbit , 1:500.

Techniques: Disruption, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot

Journal: Drug Design, Development and Therapy

Article Title: Semaglutide Alleviates Ovarian Oxidative Stress and Autophagy via the PI3K/AKT/mTOR Pathway in Mice with Polycystic Ovary Syndrome

doi: 10.2147/DDDT.S522730

Figure Lengend Snippet: (A ) Immunofluorescence images; ( B ) CYP17A1 expression area; ( C ) StAR expression area; ( D ) CYP19A1 (Immunohistochemistry); ( E ) CYP19A1 expression area. Data are presented as mean±SD. Vs NC, ## P <0.01; Vs PCOS, * P <0.05, ** P <0.01; n=6 per group.

Article Snippet: The antibodies used in the study were CYP19A1 antibodies (No. ba3704, BOSTER, China; dilution 1:50;), Beclin-1 antibodies (dilution 1:100; No.#3495, CST, USA), p62 antibodies (dilution 1:500; No. #23214, CST, USA), LC3B antibodies (dilution 1:200; No.#83506, CST, USA) was increased for half an hour and then observed.

Techniques: Immunofluorescence, Expressing, Immunohistochemistry

Journal: BMC genomics

Article Title: Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

doi: 10.1186/s12864-025-11500-5

Figure Lengend Snippet: Fig. 1 Cyp19a1 expression level detection and semen quality assessment of control group and LV-CYP19A1 group roosters. A Construction of Cyp19a1 over-expression vector. B Diagram of injection of lentivirus carrying Cyp19a1 over-expression vector into rooster testis. C qRT-PCR results of Cyp19a1 in control group and LV-CYP19A1 group. D WB results of Cyp19a1 in control group and LV-CYP19A1 group..a−b Different letters within the same row show significant differences among the groups (P < 0.05)

Article Snippet: The PVDF membranes were incubated with primary antibodies CYP19A1 (BIO-RAD, MCA2077S), CD9 (abclonal, A19027), TSG101 (abcam, ab133586), and ALIX (abclonal, A2215) proteins at 4°C overnight.

Techniques: Expressing, Control, Over Expression, Plasmid Preparation, Injection, Quantitative RT-PCR

Journal: BMC genomics

Article Title: Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

doi: 10.1186/s12864-025-11500-5

Figure Lengend Snippet: Fig. 2 Serum and seminal plasma hormone levels detection of control group and LV-CYP19A1 group roosters. Seminal plasma T (A), E2 (C), and T/E2 (E) levels of control group and LV-CYP19A1 group. Serum T (B), E2 (D), and T/E2 (F) levels of control group and LV-CYP19A1 group. a−b Different letters within the same row show significant differences among the groups

Article Snippet: The PVDF membranes were incubated with primary antibodies CYP19A1 (BIO-RAD, MCA2077S), CD9 (abclonal, A19027), TSG101 (abcam, ab133586), and ALIX (abclonal, A2215) proteins at 4°C overnight.

Techniques: Clinical Proteomics, Control

Journal: BMC genomics

Article Title: Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

doi: 10.1186/s12864-025-11500-5

Figure Lengend Snippet: Fig. 3 Morphology, particle size, and marker proteins identification of seminal plasma extracellular vesicles (SPEV). A-B Morphological characteristics of SPEV in control group and LV-CYP19A1 group. C-D Particle size distribution of SPEV in control group and LV-CYP19A1 group. E Western Blot (WB) analysis of the common SPEV marker proteins

Article Snippet: The PVDF membranes were incubated with primary antibodies CYP19A1 (BIO-RAD, MCA2077S), CD9 (abclonal, A19027), TSG101 (abcam, ab133586), and ALIX (abclonal, A2215) proteins at 4°C overnight.

Techniques: Marker, Clinical Proteomics, Control, Western Blot

Journal: BMC genomics

Article Title: Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

doi: 10.1186/s12864-025-11500-5

Figure Lengend Snippet: Fig. 5 GO and KEGG analysis of differentially expressed proteins (DEPs) between control group and LV-CYP19A1 group. A GO analysis of DEPs between control group and LV-CYP19A1 group. B KEGG analysis of DEPs between control group and LV-CYP19A1 group

Article Snippet: The PVDF membranes were incubated with primary antibodies CYP19A1 (BIO-RAD, MCA2077S), CD9 (abclonal, A19027), TSG101 (abcam, ab133586), and ALIX (abclonal, A2215) proteins at 4°C overnight.

Techniques: Control

Journal: BMC genomics

Article Title: Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

doi: 10.1186/s12864-025-11500-5

Figure Lengend Snippet: Fig. 4 Identification of differentially expressed proteins (DEPs) between control group and LV-CYP19A1 group. A Principal component analysis (PCA) of DEPs between control group and LV-CYP19A1 group. B Volcanic maps of DEPs between control group and LV-CYP19A1 group. C Heatmap of DEPs between control group and LV-CYP19A1 group

Article Snippet: The PVDF membranes were incubated with primary antibodies CYP19A1 (BIO-RAD, MCA2077S), CD9 (abclonal, A19027), TSG101 (abcam, ab133586), and ALIX (abclonal, A2215) proteins at 4°C overnight.

Techniques: Control

Journal: BMC genomics

Article Title: Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

doi: 10.1186/s12864-025-11500-5

Figure Lengend Snippet: Fig. 6 Key protein-biological process, protein-pathway, and protein–protein interaction (PPI) analysis of differentially expressed proteins (DEPs). A Key protein-biological process analysis of DEPs between control group and LV-CYP19A1 group. B PPI analysis of DEPs involved in key biological processes between control group and LV-CYP19A1 group. C Key protein-pathway analysis of DEPs between control group and LV-CYP19A1 group. D PPI analysis of DEPs involved in key pathways between control group and LV-CYP19A1 group. Orange diamonds represent the key pathways and biological processes; Green triangles represent the key proteins; Orange inverted triangles represent proteins that interact with individual key proteins; Pink octagon represent proteins that interact with multiple key proteins

Article Snippet: The PVDF membranes were incubated with primary antibodies CYP19A1 (BIO-RAD, MCA2077S), CD9 (abclonal, A19027), TSG101 (abcam, ab133586), and ALIX (abclonal, A2215) proteins at 4°C overnight.

Techniques: Control

Journal: Endocrine Connections

Article Title: 17β-Hydroxysteroid dehydrogenase type I and aromatase in ovarian cortical inclusion cysts

doi: 10.1530/EC-24-0643

Figure Lengend Snippet: Micrograph of sections of a CIC lined with mixed epithelium stained with hematoxylin and eosin. (A) CIC with flat epithelium displays regions of ciliated cells. (B) Transition from flat to mucinous epithelia is observed in CIC mix . Sections of the same cortical inclusion cyst (CIC) lined with tubal-like epithelium (CIC tube ) show immunoreactivity for: (C) 17β-hydroxysteroid dehydrogenase type 1 (HSD17B1), (D) aromatase and (E) ERα. The labeling for aromatase is lighter than that for HSD17B1 and ERα reactivity. Scale bar = 50 μm.

Article Snippet: The primary antibodies used were the HSD17B1 antibody (cat. GTX12312; GeneTex, USA), aromatase antibody H4 (cat. OASA02666; Aviva Systems Biology, USA) and Erα HC20 antibody (cat. sc-543; Santa Cruz Biotechnology, Inc., USA).

Techniques: Staining, Labeling

Journal: Endocrine Connections

Article Title: 17β-Hydroxysteroid dehydrogenase type I and aromatase in ovarian cortical inclusion cysts

doi: 10.1530/EC-24-0643

Figure Lengend Snippet: Frequency of positive immunoreactivity of 17β-hydroxysteroid dehydrogenase type 1 (HSD17B1), aromatase and ERα in the ovarian surface epithelium (OSE) and cortical inclusion cyst (CIC).

Article Snippet: The primary antibodies used were the HSD17B1 antibody (cat. GTX12312; GeneTex, USA), aromatase antibody H4 (cat. OASA02666; Aviva Systems Biology, USA) and Erα HC20 antibody (cat. sc-543; Santa Cruz Biotechnology, Inc., USA).

Techniques:

Journal: Endocrine Connections

Article Title: 17β-Hydroxysteroid dehydrogenase type I and aromatase in ovarian cortical inclusion cysts

doi: 10.1530/EC-24-0643

Figure Lengend Snippet: Frequency of positive immunoreactivity of 17β-hydroxysteroid dehydrogenase type 1 (HSD17B1), aromatase and ERα in cortical inclusion cyst (CIC) and ovarian surface epithelium (OSE) related to medically indicated oophorectomy.

Article Snippet: The primary antibodies used were the HSD17B1 antibody (cat. GTX12312; GeneTex, USA), aromatase antibody H4 (cat. OASA02666; Aviva Systems Biology, USA) and Erα HC20 antibody (cat. sc-543; Santa Cruz Biotechnology, Inc., USA).

Techniques:

Journal: Endocrine Connections

Article Title: 17β-Hydroxysteroid dehydrogenase type I and aromatase in ovarian cortical inclusion cysts

doi: 10.1530/EC-24-0643

Figure Lengend Snippet: Frequency of positive immunoreactivity of 17β-hydroxysteroid dehydrogenase type 1 (HSD17B1), aromatase and ERα in variation of cortical inclusion cyst epithelium with OSE-like (CIC ose ) or epithelium of the uterine tube-like (CIC tube ).

Article Snippet: The primary antibodies used were the HSD17B1 antibody (cat. GTX12312; GeneTex, USA), aromatase antibody H4 (cat. OASA02666; Aviva Systems Biology, USA) and Erα HC20 antibody (cat. sc-543; Santa Cruz Biotechnology, Inc., USA).

Techniques: